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ctrp3 levels  (Aviva Systems)

 
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    Structured Review

    Aviva Systems ctrp3 levels
    Melatonin increased myocardial <t>CTRP3</t> expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.
    Ctrp3 Levels, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctrp3 levels/product/Aviva Systems
    Average 86 stars, based on 1 article reviews
    ctrp3 levels - by Bioz Stars, 2026-03
    86/100 stars

    Images

    1) Product Images from "Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction"

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    Journal: Redox Biology

    doi: 10.1016/j.redox.2018.07.007

    Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.
    Figure Legend Snippet: Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.

    Techniques Used: Expressing, Immunostaining

    Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
    Figure Legend Snippet: Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Techniques Used: Expressing, Immunostaining

    Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
    Figure Legend Snippet: Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Techniques Used:

    Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
    Figure Legend Snippet: Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Techniques Used: Derivative Assay, Expressing, Cell Culture, Immunostaining, Staining, TUNEL Assay, Recombinant

    miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
    Figure Legend Snippet: miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Techniques Used: Activation Assay, Recombinant



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    Melatonin increased myocardial <t>CTRP3</t> expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.
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    Image Search Results


    Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.

    Journal: Redox Biology

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    doi: 10.1016/j.redox.2018.07.007

    Figure Lengend Snippet: Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.

    Article Snippet: Circulating CTRP3 levels were determined by commercial ELISA kit (Aviva Systems Biology, San Diego, CA) according to manufacturer's instructions.

    Techniques: Expressing, Immunostaining

    Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Journal: Redox Biology

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    doi: 10.1016/j.redox.2018.07.007

    Figure Lengend Snippet: Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Article Snippet: Circulating CTRP3 levels were determined by commercial ELISA kit (Aviva Systems Biology, San Diego, CA) according to manufacturer's instructions.

    Techniques: Expressing, Immunostaining

    Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Journal: Redox Biology

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    doi: 10.1016/j.redox.2018.07.007

    Figure Lengend Snippet: Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Article Snippet: Circulating CTRP3 levels were determined by commercial ELISA kit (Aviva Systems Biology, San Diego, CA) according to manufacturer's instructions.

    Techniques:

    Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Journal: Redox Biology

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    doi: 10.1016/j.redox.2018.07.007

    Figure Lengend Snippet: Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Article Snippet: Circulating CTRP3 levels were determined by commercial ELISA kit (Aviva Systems Biology, San Diego, CA) according to manufacturer's instructions.

    Techniques: Derivative Assay, Expressing, Cell Culture, Immunostaining, Staining, TUNEL Assay, Recombinant

    miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Journal: Redox Biology

    Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

    doi: 10.1016/j.redox.2018.07.007

    Figure Lengend Snippet: miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

    Article Snippet: Circulating CTRP3 levels were determined by commercial ELISA kit (Aviva Systems Biology, San Diego, CA) according to manufacturer's instructions.

    Techniques: Activation Assay, Recombinant

    Clinical Biochemical Characteristics of Study population.

    Journal: PLoS ONE

    Article Title: Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus

    doi: 10.1371/journal.pone.0168773

    Figure Lengend Snippet: Clinical Biochemical Characteristics of Study population.

    Article Snippet: Also, ELISA kits were used for measuring serum levels of CTRP3 (Adipogen, South Korea, Cat# AG-45A-0042EK-KI01), Adiponectin (Adipogen, South Korea, Cat# AG-45A-0001YEK-KI01) and CTRP13 (AvisceraBioscience, USA, Cat# SK00333-06).

    Techniques: Control

    (A) PBMCs gene expression of TNF-α was higher in groups II, III and IV compared to the group I (all, p < .001). (B) IL-6 PBMCs gene expression was higher in groups II, III, IV compared with the group I (all, p < .001), Also gene expression of IL-6 was higher in group IV compared with group III (p < .05). (C) PBMCs gene expression of CTRP3 was higher in control group compared with CAD group (p < .05), T2DM (p < .05) and CAD+T2DM (< .01). (D) PBMCs gene expression of CTRP13 were higher in control group compared to the CAD+T2DM group (p < .05). (E) PBMCs gene expression of adiponectin was decreased in group IV compared with group I (p < .001).

    Journal: PLoS ONE

    Article Title: Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus

    doi: 10.1371/journal.pone.0168773

    Figure Lengend Snippet: (A) PBMCs gene expression of TNF-α was higher in groups II, III and IV compared to the group I (all, p < .001). (B) IL-6 PBMCs gene expression was higher in groups II, III, IV compared with the group I (all, p < .001), Also gene expression of IL-6 was higher in group IV compared with group III (p < .05). (C) PBMCs gene expression of CTRP3 was higher in control group compared with CAD group (p < .05), T2DM (p < .05) and CAD+T2DM (< .01). (D) PBMCs gene expression of CTRP13 were higher in control group compared to the CAD+T2DM group (p < .05). (E) PBMCs gene expression of adiponectin was decreased in group IV compared with group I (p < .001).

    Article Snippet: Also, ELISA kits were used for measuring serum levels of CTRP3 (Adipogen, South Korea, Cat# AG-45A-0042EK-KI01), Adiponectin (Adipogen, South Korea, Cat# AG-45A-0001YEK-KI01) and CTRP13 (AvisceraBioscience, USA, Cat# SK00333-06).

    Techniques: Gene Expression, Control

    Univariate and multivariate analysis of the association between CTRP3 serum levels with correlated parameters.

    Journal: PLoS ONE

    Article Title: Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus

    doi: 10.1371/journal.pone.0168773

    Figure Lengend Snippet: Univariate and multivariate analysis of the association between CTRP3 serum levels with correlated parameters.

    Article Snippet: Also, ELISA kits were used for measuring serum levels of CTRP3 (Adipogen, South Korea, Cat# AG-45A-0042EK-KI01), Adiponectin (Adipogen, South Korea, Cat# AG-45A-0001YEK-KI01) and CTRP13 (AvisceraBioscience, USA, Cat# SK00333-06).

    Techniques: Gene Expression

    Univariate and multivariate analysis of the association between CTRP13 serum levels with correlated parameters.

    Journal: PLoS ONE

    Article Title: Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus

    doi: 10.1371/journal.pone.0168773

    Figure Lengend Snippet: Univariate and multivariate analysis of the association between CTRP13 serum levels with correlated parameters.

    Article Snippet: Also, ELISA kits were used for measuring serum levels of CTRP3 (Adipogen, South Korea, Cat# AG-45A-0042EK-KI01), Adiponectin (Adipogen, South Korea, Cat# AG-45A-0001YEK-KI01) and CTRP13 (AvisceraBioscience, USA, Cat# SK00333-06).

    Techniques: Gene Expression

    Odds ratios of CAD incident in Non-T2DM patients and T2DM patients according to CTRP3 and CTRP13 serum levels.

    Journal: PLoS ONE

    Article Title: Association of C1q/TNF-Related Protein-3 (CTRP3) and CTRP13 Serum Levels with Coronary Artery Disease in Subjects with and without Type 2 Diabetes Mellitus

    doi: 10.1371/journal.pone.0168773

    Figure Lengend Snippet: Odds ratios of CAD incident in Non-T2DM patients and T2DM patients according to CTRP3 and CTRP13 serum levels.

    Article Snippet: Also, ELISA kits were used for measuring serum levels of CTRP3 (Adipogen, South Korea, Cat# AG-45A-0042EK-KI01), Adiponectin (Adipogen, South Korea, Cat# AG-45A-0001YEK-KI01) and CTRP13 (AvisceraBioscience, USA, Cat# SK00333-06).

    Techniques: